Glyco-DIA

Link

Collection of Glyco-DIA Libraries-->(2.076 glycoproteins, 11.452 unique O-GalNAc type glycopeptides and five most common core 1 O-glycan structures: Tn, T, SiaTn, SiaT and diSiaT)

Glyco/DIA WorkFlow Library ID (link) Description
HEK_SC_Comprehensive Multiple MS runs (high pH reverse phase fractionated) in HCD-MS2 DDA mode, LWAC VVA enriched samples from HEK SC.
HEK_SC_Single_SingleRun Single MS run in HCD-MS2 DDA mode, LWAC VVA enriched samples from HEK SC.
HEK_WT_Comprehensive Multiple MS runs (high pH reverse phase fractionated) in HCD-MS2 DDA mode, LWAC Jacalin enriched samples from HEK293 WT.
HepG2_SC_Comprehensive Multiple MS runs (high pH reverse phase fractionated) in HCD-MS2 DDA mode, LWAC VVA enriched samples from HepG2 SC.
HepG2_SC_Secretome_SingleRun Single MS run in HCD-MS2 DDA mode, LWAC VVA enriched samples from HEK SC secretome.
HepG2_SC_SingleRun Single MS run in HCD-MS2 DDA mode, LWAC VVA enriched samples from HEK SC.
HepG2_WT_Comprehensive Multiple MS runs (high pH reverse phase fractionated) in HCD-MS2 DDA mode, LWAC Jacalin enriched samples from HepG2 WT.
HepG2_WT_SingleRun Single MS run in HCD-MS2 DDA mode, LWAC Jacalin enriched samples from HepG2 WT.
M3T4_SC_Comprehensive Multiple MS runs (high pH reverse phase fractionated) in HCD-MS2 DDA mode, LWAC VVA enriched samples from M3T4 SC.
OVCAR3_SC_Comprehensive Multiple MS runs (high pH reverse phase fractionated) in HCD-MS2 DDA mode, LWAC VVA enriched samples from OVCAR3 SC.
Serum_DIA_Comprehensive Multiple MS runs (with and without Top2 depletion, high pH reverse phase fractionated) in HCD-MS2 DDA mode, LWAC Jacalin enriched samples from human serum.
In Silico Library ID  
Original T and Tn structures expanded towards> SiaTn, SiaT and diSiaT Due to the limitation of file size on GitHub, we share all the in silico libraries on GoogleDrive